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Fish Tips logoCytocell experts share the latest and most helpful tips for fluorescence in situ hybridisation (FISH). Visit this page for timely, proven tips from the FISH experts. Choose from the sections listed below to begin viewing the related tips and tricks.

1. Reagent Storage and Disposal

Section 1 Reagent Storage and Disposal squareTips include advice on storing enzyme solution correctly, how to avoid excessive light exposure, and why you should keep light exposure of probes to a minimum.

2. Sample Preparation

Section 2 Preperation squareTips include how to optimise tissue enzyme digestion results, why you should avoid baking or aging of slides, and advice on improving cell morphology.

3. Probe Application

Section 3 Probe application squareTips include how to avoid creating a patchy hybridisation, why its a good idea to clean your slides in 70% ethanol before use, and why long pipette tips can help prevent cross contamination.

4. Denaturation

Section 4 Denaturation v2 squareTips include why you should use calibrated temperature-measuring devices and why you should test ramp up and ramp down temperature times periodically. 

5. Hybridisation

Section 5 Hybridisation squareTips include how to prevent your chromosomes appearing distorted, how to maintain optimimal results from hybridisation units, and advice if you are using an incubator for hybridisation.

6. Post Hybridisation Washes

Section 6 Post hybridisation washes v4 squareTips include advice on measuring the temperature of washing solutions and why you should rinse your slides in a dehydration series of EtOH washes.

7. Probe Analysis

Section 7 Analysis squareTips include why you should just use one type of immersion oil in your laboratory and advice on maintenance for your fluorescent microscope filter.

8. General Tips

Section 8 FISH general squareTips include the benefit of using dedicated solution jars and how to produce sharper, more distinct probe signals by following one simple step.